This kit uses highly conserved regions of SARS-CoV-2 Virus, Respiratory Syncytial Virus, Influenza A Virus and Influenza B Virus, and designs specific primer probes. The primers and probes can specifically bind to the target sequence. Under the action of reverse transcriptase and hot start Taq DNA polymerase, this segment is specifically amplified, and the fluorescent probe is hydrolyzed during amplification to generate fluorescence. The detection system contains dUTP-UDG enzyme to fully degrade potential product contamination and avoid false positive results; In addition, internal standards are set up to monitor the entire process of sample collection, transportation, nucleic acid extraction, and PCR amplification for highly conserved regions on the human genome, avoiding false negative results and ensuring the effectiveness of the entire process.
This kit can qualitatively detect the nucleic acids of SARS-CoV-2, Respiratory Syncytial Virus, Influenza A Virus, Influenza B Virus, providing information for clinical diagnosis; the detection system contains dUTP-UDG enzyme anti-contamination measures, which can avoid false positive results; At the same time, human-derived internal references are introduced to monitor the entire process of specimen collection, transportation, nucleic acid extraction, and PCR amplification to avoid false negative results; it takes only 35 minutes to complete detection and issue report.
The kit is used for the auxiliarydiagnosis and epidemiological surveillance of SARS-COV-2, RSV,Flu A and FluB infection, cannot be used as the basis for the diagnosis or exclusion of cases alone.
2. The kit can be stored for 3 days at 2-8°C after opening.
3. The kit can be stored for up to 12 months if all components are kept in the manner above. Do not use after the stated expiry date.
4. The kit can be transported in foam box sealed with ice bags or dry ice under 8°C.
