bioer PCR molecular

Sample Integrity: Our Universal Transport Medium is meticulously designed to preserve the integrity of cervical swab samples, safeguarding their quality and ensuring accurate results in diagnostic testing.

Viability Maintenance: The medium contains components that effectively maintain the viability of various microorganisms present in the sample, including bacteria, viruses, and fungi, enabling reliable detection and identification in subsequent analyses.

Stability: With optimized formulation and buffering agents, the medium provides a stable environment for sample storage and transportation, minimizing the risk of degradation and preserving the integrity of genetic material and microbial constituents.

Compatibility: Our Universal Transport Medium is compatible with a wide range of molecular and microbiological assays, facilitating diverse applications such as PCR, culture, antigen testing, and nucleic acid sequencing.

User-Friendly: The medium is supplied in convenient and ready-to-use formats, simplifying sample collection, storage, and transportation procedures for healthcare professionals and laboratory personnel.

Cervical cancer screening (e.g., HPV testing)

Sexually transmitted infection (STI) testing

Microbiological culture and identification

Molecular diagnostics (PCR, sequencing)

Epidemiological surveillance

Specimens preserved in Universal Transport Medium are allowed to be transported and / or stored at 2-8°C for up to 10 days.

Q1: The amplification efficiency is low, and there are no amplification bands in the experimental group.

A1: (1) Primers

Check if the artificially synthesized primers have degraded due to improper storage conditions; Whether the primer design is reasonable, BLAST can be used to check primer specificity or redesign primers.

(2) Template

Long term storage and repeated freezing and thawing can lead to template breakage, ring opening, or degradation. Freshly prepared DNA double strands should be used as templates; If the template GC content is too high, it will cause the double stranded DNA to be unable to open. At this time, adding PCR Enhancer (item number P021) can effectively reduce the unwinding temperature; If the template is cDNA, it is necessary to confirm the purity and integrity of the RNA used for reverse transcription.

(3) Enzymes

The enzymes used in the reaction are inactivated due to improper storage or transportation. It is recommended to replace them with new enzymes or re experiment with enzymes from another source.

(4) Amplification system

The reaction system configuration is incorrect, it is recommended to repeat the experiment.

(5) Reaction program

Check if the denaturation temperature is accurate and if the PCR instrument indicates a temperature that matches the actual temperature. If the temperature is too high, the enzyme will quickly become inactive in the first few cycles, while if the temperature is too low, the template denaturation will not be complete; The annealing temperature is not suitable. A gradient can be set for the annealing temperature to explore a suitable annealing temperature; Set annealing temperature based on primer Tm value, such as primer Tm value≥ At 72 ℃, the annealing step can be removed and the subsequent extension step (two-step PCR) can be carried out directly.

Q2: The brightness of the amplified bands is not very bright.

A2: (1) Primers

Check if the artificially synthesized primers have degraded.

(2) Template

Firstly, confirm the quality of the template. Long term storage and repeated freeze-thaw cycles can cause template breakage, ring opening, or degradation. Freshly prepared DNA double strands should be used as the template; If the template is not available, the first amplification product can be diluted in multiples and used as a template for secondary amplification.

(3) Reaction program

You can try using the Touch Down program; Extend the extension time and increase the number of cycles.

Product Details FAQ Specification Instructions