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Avian influenza (AI) viruses, commonly known as bird flu, are members of the family orthmyxoviridae, Type A which is subdivided into categories (strains) depending on the outer proteins H (Haemagglutinin) and N (Neuraminidase).  There are many types of avian influenza, which are all caused by various strains of type A influenza virus (e.g., H5N1, H7N3, H9N2). Avian influenza A(H5N1) illness is caused by the avian influenza A(H5N1) virus. The disease spreads by direct contact or through contaminated faeces and bodily fluids. New AI virus strains are created frequently which means that there is a constant risk that one of the new strains may spread easily among people. Avian Influenza continues to be a threat to the worldwide poultry industry. An effective control strategy for avian influenza in domesticated poultry is therefore an essential element in the protection of both bird and human populations. A laboratory test is required to diagnose Avian influenza (AI) viruses, Real-Time PCR technology has the advantages of high specificity and sensitivity, so it is recommended for laboratory detection. Based on its mature molecular diagnosis platform and technical reserves, Bioer Technology has developed related products of avian influenza virus nucleic acid detection reagents.

Product features

Sample Type: Animal plasma, serum, swab, tissue, and virus culture can be selected.

High Sensitivity: The detection limit can reach 200 copies/ml.

High Specificity: It Can effectively detect Avian Influenza virus, and no cross-reaction with Influenza B Virus, Newcastle Disease Virus, Infectious Bursal Disease Virus, Avian Infectious Bronchitis Virus, Avian Infectious Laryngeal Tracheitis Virus, Avian Maorik's Disease Virus, Chicken Mycoplasma, Salmonella, Chicken Genomic DNA.

Wide Applicability: It Can be compatible with various fluorescent quantitative PCR instruments on the market, especially Bioer Real-Time PCR instruments.

Good Stability: The reagent kit adopts the form of lyophillization. After lyophillization, the active substances (such as enzymes) in the product will be protected at ambient temperature, and can be transported at room temperature, improving and extending the stability of the reagent, greatly reducing the problems of reagent performance degradation or failure caused by temperature changes.

Simple Operation and Short Time Consumption: The reagent kit does not need to be prepared, and after re-dissolution, it only needs to be packaged in one step to add samples for testing, and the test results can be obtained within 1 hour.

Product parameters
Experimental data

The standard curve was drawn after using the kit to detect the positive samples of avian influenza virus. The correlation coefficient of Ct value of the target gene of avian influenza virus was 1.000, indicating that the kit had a good linear relationship and high PCR efficiency. The results are shown in the following figure:

H1N1, H3N2, H5N1, H7N9, H9N2, Influenza B Virus, Newcastle Disease Virus, Infectious Bursal Disease Virus, Chicken Infectious Bronchitis Virus, Avian Infectious Laryngeal Tracheitis Virus, Chicken Maorik's Disease Virus, Chicken Mycoplasma, Salmonella and Chicken genomic DNA were detected by using the kit. The results showed that: Except for H5N1, H7N9 and H9N2, the other samples were negative, indicating that the kit did not cross with other viruses and had good specificity.

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