
Mycoplasma Nucleic Acid Detection Kit (Fluorescent PCR)
Mycoplasma contamination is a critical issue in the biopharmaceutical industry, warranting stringent prevention and control measures. Contamination can result in abnormal cell cultures, compromised quality of biopharmaceutical products, and, in severe cases, pose safety risks that jeopardize the success rate and compliance of drug development and manufacturing processes.
Details
Product Description
The Bioer Mycoplasma Nucleic Acid Detection Kit (Fluorescent PCR) utilizes polymerase chain reaction (PCR) combined with TaqMan fluorescent probe technology. Primers and probes are designed within the conserved 16S region, validated in accordance with the requirements outlined in Chapter 2.6.7 of the European Pharmacopoeia and other relevant mycoplasma detection standards. This kit enables qualitative detection of nucleic acids from over 200 species of mycoplasma and spiroplasma. With high sensitivity and broad sample compatibility, the kit incorporates a non-competitive exogenous internal control to monitor the entire extraction and detection process, ensuring accurate and reliable results. As an ideal choice for laboratory mycoplasma contamination testing, it supports quality control in biopharmaceutical research and production, providing robust protection for sample safety.
Product Features
Simplified Operation:
- Pre-mixed PCR reaction solution in a one-tube format eliminates the need for additional preparation, streamlining the workflow.
Robust Quality Control:
- Incorporates an exogenous internal control to monitor the entire extraction and amplification process, minimizing the risk of false-negative results.
Anti-Contamination System:
- Includes dUTP/UDG components to effectively degrade PCR product contamination and prevent false-positive results.
Strong Specificity:
- No cross-reactivity with over 20 common pathogenic microorganisms, ensuring accurate and reliable results.
Storage Conditions
-25℃~-15℃
Specification
| Sample Type | Cell cultures, biological products. |
|---|---|
| Method | qPCR |
| Sensitivity | ≤10 CFU/mL |
| Number of Species | >200 species |
| Internal Control | Exogenous internal control, positive control, negative control |
| Run Time | 50 min |
| Compatible Platform | LineGene 9600, QuantGene 9600, FQD-A1600, and ABI 7500 |
| Recommended Purification Kit | BSC86 MagaBio plus Virus DNA/RNA Purification Kit Ⅲ |
| Storage Condition | -20 ± 5 ℃ |
Ordering
| Cat# | Product Name | Packing size |
|---|---|---|
| BSJ52M1 | Mycoplasma Nucleic Acid Detection Kit (Fluorescent PCR) | 50T |
| BSJ52L1 | Mycoplasma Nucleic Acid Detection Kit (Fluorescent PCR) | 100T |
