
PEDV Real Time PCR Detection Kit
Porcine epidemic diarrhea virus (PEDV) belongs to the genus Alpha coronaviruses of the coronaviruses family. Porcine epidemic diarrhea (PED), a digestive or respiratory tract disease that is highly infectious and pathogenic in pigs, occurs frequently in the cold season of late winter and early spring, causing huge economic losses and seriously endangering the pig industry in China and the world.
Details
Product Description
This kit designs primer probes in specific conserved areas of porcine epidemic diarrhea virus, and uses polymerase chain reaction (PCR) combined with Taqman fluorescence probe technology to achieve qualitative detection of pathogens, aiming to establish a fluorescence quantitative PCR detection method that can rapidly diagnose porcine rotavirus, and achieve the purpose of rapidly shunting pigs with related suspected symptoms, in order to effectively prevent and control swine rotavirus infection diseases.
Product Features
1. Applicable samples: Pig intestinal contents, mesenteric lymph nodes, live pig excreta, etc.
2. Strong specificity: No cross-reaction with foot-mouth disease virus type O /A/Asian Type 1, Porcine Reproductive and Respiratory Syndrome, porcine Rotavirus, porcine transmissible gastroenteritis virus, porcine Mycoplasma pneumoniae, porcine circovirus, porcine pseudo rabies virus, porcine parvovirus and classical classical swine fever virus.
3. High accuracy: Effectively identify porcine epidemic diarrhea virus, detection results in line with expectations.
4. Simple operation: Complete the RT-PCR process in one step, complete closed tube amplification and detection, and prevent aerosol pollution.
Application Field
The kit is used for the detection of Porcine Epidemic Diarrhea Virus (PEDV) in intestinal contents, mesenteric lymph nodes, excreta of pigs. The kit for research only, cannot be used alone as the sole criteria for diagnosing or excluding cases.
Storage Conditions
1. The kit need to be transported under freezing conditions.
2. The kit should be stored at -25℃~-15℃ away from light, and avoid repeated freeze-thaw more than 5 times.
3. The kit can be stored for up to 12 months if all components are kept in the manner above. (Please use the kit in the period of validity).
Specification
| Parameter name | Parameter description |
|---|---|
| Sample type | Pig intestinal contents, mesenteric lymph nodes, live pig excreta, etc |
| Precision | The variation coefficients of precision between batches, within batches, between days and within days were all less than 3% |
| Accuracy | Porcine epidemic diarrhea virus can be accurately detected |
| Specificity | There was no cross-reaction with foot-mouth disease virus type O /A/Asian type 1, porcine blue ear virus, porcine Rotavirus, porcine transmissible gastroenteritis virus, porcine Mycoplasma pneumoniae, porcine circovirus, porcine pseudo rabies virus, porcine parvovirus and classical classical swine fever virus. |
| Supporting instrument | Bioer LineGene, QuantGene series fluorescence PCR instrument |
| Detection time | The detection can be completed within 1 hour |
| Preservation condition | Store at -20 ± 5 ℃ away from light |
Ordering
| Cat# | Description | Packing size |
|---|---|---|
| BSL34S1 | PEDV Real Time RT-PCR Detection kit | 25T |
| BSL34M1 | PEDV Real Time RT-PCR Detection kitPCR) | 50T |
