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PCR Enzymes and Mix
PCR Enzymes and Mix
BioRT All-in-One RT Master Mix for qPCR（+gDNA Wiper）
This kit is a new-generation qPCR-specific reverse transcription kit. The product integrates all components required for genomic DNA removal and RNA reverse transcription into a single tube. Simply add template and water to initiate the reaction, which can be completed in as little as 5 minutes. The resulting cDNA is compatible with both dye-based and probe-based qPCR methods.
BioRT Real Time RT-PCR Kit（SYBR Green）
The BioRT Real Time RT-PCR Kit (SYBR Green) is designed for efficient one-step fluorescent quantitative PCR detection using RNA as the template. This kit enables precise and reliable real-time monitoring of PCR amplification with SYBR Green dye, which binds specifically to double-stranded DNA. It offers a streamlined workflow, making it ideal for gene expression analysis, viral RNA detection, and other RNA-based research applications.
BioEasy Universal qPCR Master Mix (SYBR Green)
This kit utilizes nucleic acid amplification technology combined with SYBR Green for Real-Time PCR, enabling rapid and accurate detection and quantification of target genes. The kit provides a 2× Universal SYBR Green Mix, requiring only the addition of primers and template to perform Real-Time PCR reactions, making it fast and easy to operate.
BioEasy 2×qPCR Master Mix (Lyophilizable)
BioEasy 2×qPCR Master Mix (Lyophilizable) is a reagent kit specially developed for the lyophilization process, designed for conducting quantitative fluorescence PCR (qPCR) reactions using DNA as a template. This product contains an antibody-modified hot-start Taq DNA polymerase, which effectively suppresses nonspecific primer amplification at low temperatures, thereby enhancing the specificity of the amplification reaction.
BioRT Master HiSensi cDNA First Strand Synthesis kit
This kit is designed for RNA reverse transcription experiments in the field of biochemical molecular research. It can convert various types of RNA (including total RNA, mRNA, tRNA, rRNA) into cDNA to meet the requirements for synthesizing cDNA of different lengths.
BioEasy Master Mix (SYBR Green)
This kit contains one kind of efficient hot start and high confidence DNA polymerase, and some specific PCR enhancers. So efficiency of PCR reaction and specificity, sensitivity and repeatability of Real-time PCR are greatly improved. BioEasy Master Mix (SYBR Green) Kit is convenient premix of the components (except primers, template and water) necessary to perform Real-time PCR using SYBR Green Dye. Users just add Primers and template into the system, when they perform quantity assay or melt curve assay.
BioReady Heat-labile UDG
BioReady Thermosensitive Uracil-DNA Glycosylase (UDG) is derived from recombinant cloning and expression in Escherichia coli. It effectively hydrolyzes uracil (U) in single-stranded or double-stranded DNA longer than 6 base pairs, with no activity on RNA. Its primary application is to prevent contamination of PCR amplification products. It works by replacing dTTP with dUTP in the PCR reaction, creating PCR amplification products containing dU bases. UDG then cleaves the dU bases in these amplification products, creating abasic sites, preventing the DNA strand from serving as a template for subsequent PCR, thus achieving the goal of preventing PCR product contamination. Additionally, this thermosensitive UDG becomes inactive in the subsequent 50°C reverse transcription or 95°C pre-denaturation steps without affecting the amplification of the template.
BioEasy 2xRT-qPCR Master Mix (Lyophilizable)
BioEasy 5×qPCR Master Mix (Lyophilizable) is specifically designed and developed to facilitate lyophilization for fluorescent quantitative PCR products. It contains the novel fast hot-start enzyme BioQuick Hot Start Taq, which effectively suppresses non-specific amplification, thereby enhancing the specificity of the amplification reaction.
BioRT Master One Step RT-PCR Kit Ⅲ (with dye)
This kit provides a one-step reverse transcription PCR (RT-PCR) reagent. Before the reaction, you only need to add primers and templates. The optimized reaction system allows RT and PCR to be carried out in the same reaction, reducing the risk of contamination during processes like opening and pipetting.
Cell Culture Consumables
Cell Culture Consumables