The kit is supplied with both oligo(dT)and random primer, In addition, customers can also use gene specific primers, random primers initiate cDNA synthesis at multiple random sites along RNA, ensuring unbiased representation. Oligo(dT) primers specifically prime cDNA synthesis from the poly (A) tail of mRNA, enabling selective amplification of mRNA transcripts. The benifits allows for unbiased initiation of cDNA synthesis from RNA, providing comprehensive coverage of the transcriptome and selective amplification of mRNA, enabling accurate analysis of gene expression.
- Efficient synthesis of first strand cDNA up to 14 kb.
- Oligo (dT) and random primer are included with the kit.
- 30 min to complete cDNA synthesis.
- A modified Moloney Murine Leukemia Virus reverse transcriptase (M-MLV) was added with enhanced template affinity and reduced RNase H activity.
First strand cDNA synthesis for RT-PCR and RT-qPCR.
Construction of full length cDNA libraries.
