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Influenza A Virus/Influenza B Virus Nucleic Acid Detection Kit(Fluorescence RT-PCR)

Influenza A Virus/Influenza B Virus Nucleic Acid Detection Kit(Fluorescence RT-PCR)

Respiratory Tract Infections Diseases

Influenza is an acute respiratory infection caused by influenza virus. At present, the influenza viruses that infect humans are mainly influenza A and B viruses. Both are infectious and harmful, and their clinical symptoms are similar after infection.The kit can be used to detect two kinds of influenza virus nucleic acid in one test, and has the features of short time, good specificity and high sensitivity. In addition, the kit add a human internal reference to monitor the entire process of sample collection, transportation, nucleic acid extraction and PCR amplification to ensure the effectiveness of the experiment.The kit can be applied to Bioer’s Fluorescence Quantitative Detection System, LineGene 9600 Plus (FQD-96A) and QuantGene 9600（FQD-96C）. The instrument should contain at least three channels of FAM, HEX (VIC/JOE) and CY5.

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Product Description

Influenza A Virus /Influenza B Virus Nucleic Acid Detection Kit (FluorescenceRT-PCR) amplifies and detects viral RNA in swab specimens obtained fromsuspected patients. This product selects the M gene (FAM) region of Influenza AVirus and N gene (HEX) regions of lnfluenza B Virus, and designs two sets ofprimers and fluorescent probes that cover two sites of the genes. The two sets ofprimers and probes can specifically bind to the target sequences. When the RT-PCR amplification reaction is performed, the fluorescent signal(s) can be detected by afull-automatic fluorescent PCR detector to realize real-time online monitoring ofthe RT-PCR reaction. In order to control the entire extraction and detection process,human Ribonuclease gene (CY5) was act as a non-competitive internal controlduring the extraction and detection process.

Product Features

Strong applicability: suitable for Oropharyngeal Swabs

High sensitivity: up to 200 copies/mL

High accuracy: The kit can simultaneously detect influenza A virus subtypes: A H1N1, A H1N1 (2009), A H3N2, A H5N1, A H7N9; influenza B virus: Victoria strain and Yamagata strain. The positive and negative coincidence rates were 100% and 100% respectively.

Internal Control Included: Incorporates an internal control to monitor the entire extraction and detection process, ensuring

reliability and quality of the results.

Application Field

Respiratory infection disease detection

Storage Conditions

The kit can be stored for 3 days at 2-8 ℃ after opening

Ordering Information

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Streptococcus pneumoniae and Haemophilus influenzae are two major bacterial pathogens responsible for respiratory infections, including pneumonia, meningitis, and otitis media. Streptococcus pneumoniae, also known as pneumococcus, is a gram-positive bacterium that can cause severe diseases, particularly in young children, the elderly, and immunocompromised individuals. Haemophilus influenzae, a gram-negative bacterium, is similarly associated with respiratory tract infections and can lead to invasive diseases such as bacteremia and epiglottitis. Both bacteria are spread through respiratory droplets, and vaccination is available to prevent infections caused by these pathogens.