Avian Influenza Virus H5N1 Subtype Nucleic Acid Detection Kit (Fluorescence PCR)

Product Description

The kit selects specific conserved regions of AIV H5N1 subtype to design primers and probes. The detection system uses two different fluorophores to independently detect AIV H5 subtype target gene (FAM channel) and AIV N1 subtype target gene (HEX channel).

Product Features

• Sample Types: Throat swabs, cloacal swabs, serum, and lung tissue.

• Ultra-High Sensitivity: Detection limit as low as 500 copies/mL.

• High Specificity: Enables accurate differentiation of avian influenza virus subtypes while maintaining excellent negative results for influenza B virus testing.

• Broad Compatibility: Compatible with a wide range of commercially available real-time PCR systems.

• Easy Operation & Rapid Results: The ready-to-use premix format simplifies workflow with fewer components, and the complete PCR reaction can be completed within 1 hour.

Application Field

This kit can be used for qualitative testing of avian influenza virus（AIV) H5N1 subtype in throat swabs, cloaca swabs, serum and lung tissue of suspected infection or other animals that require the diagnosis or differential diagnosis of avian influenza virus H5N1 subtype infection (chicken, duck, goose, etc.).

Application Cases

Case 1: Use this kit to test positive samples of the H5N1 virus and draw a standard curve.It was found that the correlation coefficient of the CT values detected by each subtype reached more than 0.995, indicating that the linear relationship of the kit is good and the PCR efficiency is high. The results are as follows:

Case 2: Specific detection of avian influenza virus H5N1 subtype nucleic acid detection kit Positive samples of H1N1, H3N2, H5N1, H7N9, H9N2, Type B V, Type B Y, Newcastle disease virus, duck fever virus, chicken infectious Farsi virus, chicken infectious bronchitis virus were tested using a kit. The results showed that the H5N1 subtype of avian influenza could detect H5 subtype and N1 subtype samples, and the rest of the samples were negative; It shows that the kit has no crossover with the above viruses and has good specificity. The results are summarized as follows:

Case 3: The kit was placed at -20ºC and 37ºC for 7 days, and after being removed, it was used to detect positive samples of different concentrations. The results showed that: after the kit was placed at 37ºC for 7 days, when the reagent placed at -20ºC was used to detect samples of different concentrations at the same time, the detection effect was not significantly different, indicating that the stability of the kit is good. The detection effect is as follows.

Storage Conditions

1. The kit need to be transported under freezing conditions.

2. The kit should be stored at -25℃~-15℃ away from light, and avoid repeated freeze-thaw more than 5 times.

3. The kit can be stored for up to 12 months if all components are kept in the manner above. (Please use the kit in the period of validity).