Avian Influenza Virus H5N8 Subtype Nucleic Acid Detection Kit (Fluorescence PCR)
Details
Product Description
The kit selects specific conserved regions of AIV H5N8 subtype to design primers and probes. The detection system uses two different fluorophores to independently detect AIV H5 subtype target gene (FAM channel) and AIV N8 subtype target gene (HEX channel).
Product Features
- Sample Types: Throat swabs, cloacal swabs, serum, and lung tissue.
- Ultra-High Sensitivity: Detection limit as low as 500 copies/mL.
- High Specificity: Enables accurate differentiation of avian influenza virus subtypes while maintaining excellent negative results for influenza B virus testing.
- Broad Compatibility: Compatible with a wide range of commercially available real-time PCR systems.
- Easy Operation & Rapid Results: The ready-to-use premix format simplifies workflow with fewer components, and the complete PCR reaction can be completed within 1 hour.
Application Field
This kit can be used for qualitative testing of avian influenza virus (AIV) H5N8 subtype in throat swabs, cloaca swabs, serum and lung tissue of suspected infection or other animals that require the diagnosis or differential diagnosis of avian influenza virus H5N8 subtype infection (chicken, duck, goose, etc.).
Application Cases
Using this kit to detect the H5N8 positive sample and make standard curve, which find the correlation coefficients of the Ct values detected by each subtype are above 0.995, indicating that the kit has a good linear relationship and high PCR efficiency. The results are as follows:
The kit was stored at -20ºC and 37ºC for 7 days, and was taken out to detect positive samples of different concentrations. The results showed that the kits being stored at 37ºC for 7 days, compared to the kits being stored at -20ºC to detected samples of different concentrations at the same time, there was no significant difference in the detection effect, indicating that the stability of the kit was good. The detection result is as follows:
Storage Conditions
1. The kit need to be transported under freezing conditions.
2. The kit should be stored at -25℃~-15℃ away from light, and avoid repeated freeze-thaw more than 5 times.
3. The kit can be stored for up to 12 months if all components are kept in the manner above. (Please use the kit in the period of validity).
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