Avian Influenza Virus H7N9 Subtype Nucleic Acid Detection Kit (Fluorescence PCR)
Details
Product Description
The kit selects specific conserved regions of AIV H7N9 subtype to design primers and probes. The detection system uses two different fluorophores to independently detect AIV H7 subtype target gene (FAM channel) and AIV N9 subtype target gene (HEX channel).
Product Features
- Sample Types: Throat swabs, cloacal swabs, serum, and lung tissue.
- Ultra-High Sensitivity: Detection limit as low as 500 copies/mL.
- High Specificity: Enables accurate differentiation of avian influenza virus subtypes while maintaining excellent negative results for influenza B virus testing.
- Broad Compatibility: Compatible with a wide range of commercially available real-time PCR systems.
- Easy Operation & Rapid Results: The ready-to-use premix format simplifies workflow with fewer components, and the complete PCR reaction can be completed within 1 hour.
Application Field
This kit can be used for qualitative testing of avian influenza virus（AIV） H7N9 subtype in throat swabs, cloaca swabs, serum and lung tissue of suspected infection or other animals that require the diagnosis or differential diagnosis of avian influenza virus H5N8 subtype infection (chicken, duck, goose, etc.).
Application Cases
Positive samples of H1N1, H3N2, H5N1, H7N9, H9N2, Type B V, Type B Y, Newcastle disease virus, duck fever virus, chicken infectious Farsi virus, and chicken infectious bronchitis virus were tested using a kit. The results showed that the H7N9 subtype of avian influenza can detect H7 subtype and N9 subtype samples, and the rest of the samples were negative; it shows that the kit has no cross with the above viruses and has good specificity. The results are summarized as follows:
The positive samples of avian influenza virus H7N9 were repeatedly tested with this kit 20 times. After statistics, it was found that the CT value coefficient of variation (CV) of the test results of each subtype was less than 1%, indicating that the precision of the kit is good, and the test results of the same sample are stable and reliable. The specific results are summarized as follows：
Storage Conditions
1. The kit need to be transported under freezing conditions.
2. The kit should be stored at -25℃~-15℃ away from light, and avoid repeated freeze-thaw more than 5 times.
3. The kit can be stored for up to 12 months if all components are kept in the manner above. (Please use the kit in the period of validity).
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