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Avian Influenza Virus Lyophilized Nucleic Acid Detection Kit (Fluorescence PCR)

Avian Influenza Virus Lyophilized Nucleic Acid Detection Kit (Fluorescence PCR)

Avian influenza (AI) viruses, commonly known as bird flu, are members of the family orthmyxoviridae, Type A which is subdivided into categories (strains) depending on the outer proteins H (Haemagglutinin) and N (Neuraminidase). There are many types of avian influenza, which are all caused by various strains of type A influenza virus (e.g., H5N1, H7N3, H9N2).

Details

Product Description

In this kit, RNA viruses synthesize cDNA strands using RNA as templates under the action of reverse transcriptase. Qualitative detection of Avian influenza virus by polymerase chain reaction (PCR) combined with Taqman fluorescence probe technique under the action of polymerase.

Product Features

- Multiple Sample Types: Plasma, serum, swabs, tissue samples, and virus cultures.

- High Sensitivity: Detection limit down to 200 copies/mL.

- Room Temperature Stability: Lyophilized format enables convenient storage and transportation.

- Fast & Simple Workflow: Ready-to-use design delivers reliable results within 1 hour.

Application Field

The Avian Influenza Virus Lyophilized Nucleic Acid Detection Kit (Fluorescence PCR) is utilized in veterinary diagnostics for rapid detection of avian influenza viruses in poultry and wild bird populations, aiding in surveillance and outbreak management.

Application Cases

The standard curve was drawn after using the kit to detect the positive samples of avian influenza virus. The correlation coefficient of Ct value of the target gene of avian influenza virus was 1.000, indicating that the kit had a good linear relationship and high PCR efficiency. The results are shown in the following figure:

avian-influenza-virus-lyophilized-nucleic-acid-detection-kit-fluorescence-pcr-detail-2.jpg

Storage Conditions

1. The unopened kit should be stored at -25℃~8℃ away from light.

2. The kit can be stored for up to 12 months if all components are kept in the manner above. Do not use after the stated expiry date.

3. Don’t open aluminum pouch unless you are ready to use the kit.

4. The lyophilized reagents (reaction mixture) reconstituted with water should be stored at -25℃~-15℃ away from light for up to 1 week. Avoid repeated freeze-thaw (no more than 2 times).

5. The kit can be transported at up to 37℃ for no more than 1 month.

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