BioRT One-step RT-qPCR Kit V
BioRT One-step RT-qPCR Kit V is designed for one-step real-time RT-qPCR applications, integrating reverse transcription and PCR amplification in a single reaction. The kit contains 5× One-step RT Mix V, enabling higher template input volume to improve detection sensitivity, especially for low-copy RNA targets.
Details
Product Description
BioRT One-step RT-qPCR Kit V is designed for one-step real-time RT-qPCR applications, integrating reverse transcription and PCR amplification in a single reaction. The kit contains 5× One-step RT Mix V, enabling higher template input volume to improve detection sensitivity, especially for low-copy RNA targets.
The optimized enzyme system combines high-efficiency reverse transcriptase with antibody-modified hot-start Taq DNA polymerase, delivering enhanced sensitivity and specificity. The built-in heat-labile UDG system effectively prevents carryover contamination by degrading residual PCR products, ensuring reliable and reproducible results.
Product Features
One-Step RT-qPCR
- Reverse transcription and qPCR amplification in a single tube for a streamlined workflow.
High Sensitivity & Specificity
- Hot-start Taq polymerase technology enables reliable and accurate detection.
UDG Carryover Prevention
- Reduces contamination risk from previous amplification products.
Wide Instrument Compatibility
- Compatible with most real-time PCR instruments.
Storage Conditions
1. The kit should be transported in foam box sealed with ice bags or dry ice at 2-8℃ or lower.
2. Kit storage: Store in a -25~-15°C refrigerator away from light, and the repeated freezing and thawing should not exceed 5 times.
3. Validity period: 12 months, please use within the validity period.
Ordering
| Cat # | Product Name | Packing Size |
|---|---|---|
| BSB145S1 | BioRT One-step RT-qPCR Kit V | 100T |
| BSB145M1 | BioRT One-step RT-qPCR Kit V | 500T |
| BSB145L1 | BioRT One-step RT-qPCR Kit V | 5000T |
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