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BioEasy Multiplex qPCR Probe Master MixⅠ (UDG plus)

BioEasy Multiplex qPCR Probe Master MixⅠ (UDG plus)

This kit is a ready-to-use hot start qPCR probe master mix, the mix containing the optimized PCR buffer and antibody-based hot start Taq enzyme, the activity of Taq DNA polymerase is blocked by antibody before pre-denaturation, and its activity can be rapidly released under high temperature conditions, saving the running time of fluorescent PCR and improving the sensitivity and specificity of amplification.

Details

Product Description

Multiplex qPCR Probe Master Mix I (UDG plus) (2×) is designed as an ready-to-use solution for detection and quantitation of target DNA sequences using hydrolysis probes. The combination of optimized buffer, hot-start Taq DNA polymerase and stabilizers results in high amplification efficiency, high detection sensitivity and less non-specific amplification in real-time PCR analyses. This pre-mixed formulation allows us just adding primers, probes and templates, which reduce number of steps and save time.

Product Features

- Simple & Convenient: Ready-to-use qPCR mix simplifies reaction setup and minimizes the risk of contamination.

- Carryover Contamination Prevention: The dUTP/UDG system effectively prevents false-positive results caused by PCR product carryover.

- High Specificity & Sensitivity: Antibody-modified hot-start Taq DNA polymerase minimizes non-specific amplification for sensitive and specific detection.

- Broad Applicability: The optimized buffer system ensures efficient amplification of diverse targets and supports both singleplex and multiplex qPCR assays.

Application Field

Genotyping

Gene Expression Analysis

Storage Conditions

-20 ℃