BioReady Taq Mix /BioReady Taq Mix（with dye）
Details
Product Description
The reagent kit is available with or without a dye indicator, providing flexibility for customer needs. The version with a dye indicator, BioReady Taq Mix (with dye), slifies the process as it only requires the addition of templates and primers. The resulting PCR products can be directly analyzed by gel electrophoresis, making it a convenient and straightforward procedure.
Product Features
- High adaptability: suitable for amplification from samples of different sources, including high G/C content.
- Fast Amplification Speed: 5s/kb.
- Amplicon length (up to kb): ＜8Kb
- Multiplex PCR: up to 12-plex detection
Application Cases
1. The experiment was conducted using BioReady Taq Mix to amplify fragments of different origins and lengths. The results of agarose gel electrophoresis are shown in the following figure: 1-3: Yeast, Shiitake mushroom, Oyster mushroom gDNA; 4-6: Feline herpesvirus, Pseudorabies virus, Avian Chlamydia gDNA; 7: Mouse cDNA; 8-9: Arabidopsis gDNA; 10-11: Human gDNA; 12-13: Lambda DNA; 14: Escherichia coli gDNA.
Results: The results show that BioReady Taq Mix exhibits good amplification capabilities and versatility, making it suitable for PCR amplification of various templates, including animals, plants, fungi, bacteria, viruses, and more.
2. The experiment involved diluting high-concentration Escherichia coli DNA to concentrations of 0.4 ng, 0.2 ng, 0.1 ng, and 0.01 ng (as labeled in the gel image: 1-4). Different DNA amplification reagents, including BioReady Taq Mix from Bioer, as well as three competitive products (labeled as Vendor 1-3), were used to amplify a 16S target gene segment (1470 bp) to assess the sensitivity of the reagents.
Results: The results indicate that BioReady Taq Mix from Bioer can consistently amplify the target gene segment from genomic templates with concentrations as low as 0.01 ng. This demonstrates that its sensitivity is superior to the competitive products used in the experiment.
Related Products
You May Also Like
BioRT All-in-One RT Master Mix for qPCR（+gDNA Wiper）
This kit is a new-generation qPCR-specific reverse transcription kit. The product integrates all components required for genomic DNA removal and RNA reverse transcription into a single tube. Simply add template and water to initiate the reaction, which can be completed in as little as 5 minutes. The resulting cDNA is compatible with both dye-based and probe-based qPCR methods.
BioQuick Hot Start Taq DNA Polymerase V1
This product is a novel antibody-coated hot-start enzyme developed by our company. The coated antibody rapidly deactivates at 95°C within 1 minute, allowing Taq enzyme to regain activity. This feature significantly improves the suppression of nonspecific reactions caused by primer mis-annealing or primer-dimers during the PCR system setup and amplification process, resulting in excellent performance in multiple amplifications.
BioReady rTaq
rTaq is a modified form of the Taq DNA polymerase enzyme that is commonly used in molecular biology applications, particularly in the polymerase chain reaction (PCR) technique. The "r" in rTaq stands for "recombinant," indicating that it is produced using recombinant DNA technology. This modified enzyme exhibits improved stability at higher temperatures compared to the native Taq polymerase, allowing for more efficient DNA amplification in PCR reactions. rTaq is widely utilized in various research and diagnostic settings due to its robust performance, high fidelity, and versatility in amplifying target DNA sequences.