BioRT One-step RT-qPCR Kit (UDG plus)
Details
Product Description
This kit enables reverse transcription and real-time PCR amplification in a single tube, simplifying the workflow and reducing contamination risks. The optimized enzyme and buffer systems ensure efficient template amplification with high sensitivity and specificity. A dUTP/UDG system effectively prevents carryover contamination without interfering with amplification, ensuring reliable and consistent performance.
Product Features
- High Specificity: Utilizes a hot-start method to effectively control non specific reactions.
- Contamination Prevention: The qPCR premix contains dUTP/UDG components to prevent false positives caused by PCR product contamination.
- Wide Applicability: Suitable for probe-based assays.
- Simple Operation: Few components make it easy to use.
- Multiplexing: Supports multiple PCR reactions.
Application Cases
The PEDV control sample was subjected to gradient dilution using sample preservation solution, and then extracted using the MagaBio plus Virus Nucleic Acid Purification Kit III. The extracted material was subsequently used to compare and verify amplification performance and Ct values using the reagents from BSB73 and competing manufacturers N and T.
Results: The results show that in the amplification performance of PEDV using BioEasy's BioRT One-Step RT-qPCR Kit (containing UDG) with PEDV primer-probe detection, The amplification curves and Ct values of BSB73 are superior to those of the competitor products from foreign suppliers T and N.
Storage Conditions
1. The kit needs to be transported under frozen conditions.
2. Kit storage: Store in a -20°C refrigerator away from light, and the repeated freezing and thawing should not exceed 5 times.
3. Validity period: 12 months, please use within the validity period.
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