Dengue Virus/Zika Virus/Chikungunya Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Dengue virus (DENV), Zika virus (ZIKV), and Chikungunya virus (CHIKV) are mosquito-borne pathogens widely distributed in tropical and subtropical regions, causing similar symptoms such as fever, rash, headache, nausea, and muscle/joint pain. DENV has four serotypes and belongs to the Flaviviridae family; ZIKV is also Flaviviridae and can be transmitted via mosquitoes, mother-to-child, sexual contact, and blood; CHIKV belongs to the Togaviridae family and is mainly spread by Aedes aegypti and Aedes albopictus.
Details
Product Features
Easy Operation
– Pre-mixed reagent design minimizes sample preparation time and streamlines the workflow.
Multiplex Detection
– Simultaneous qualitative detection of ZIKV, DENV (DENV-1, DENV-2, DENV-3, DENV-4), and CHIKV RNA in a single reaction, improving detection efficiency.
Internal Control
– Human β-globin gene serves as an internal control to monitor nucleic acid extraction and PCR amplification efficiency, ensuring reliable results and reducing false negatives.
High Sensitivity
– Limit of detection as low as 500 copies/mL, enabling accurate identification of low-concentration pathogens and minimizing missed diagnoses.
Product Description
This kit enables simultaneous qualitative detection of DENV, ZIKV, and CHIKV in a single assay. It employs the human β-globin gene as a universal internal control system—serving as an endogenous control for human-derived samples and an exogenous control for mosquito-derived samples. The internal control monitors the entire workflow from sample collection and transportation to nucleic acid extraction and detection, effectively minimizing the risk of false-negative results.
Storage Conditions
-25℃ ~-15 ℃
Specification
| Sample Type | Human serum, plasma, whole blood, cerebrospinal fluid, tissue cultures, viral cultures and mosquito-borne samples |
|---|---|
| Sensitivity | 500 copies/mL |
| Precision | The coefficients of variation for inter-batch, intra-batch, inter-day, and intra-day precision are all less than 5%. |
| Internal Control | β-globin |
| Specificity | No cross-reactivity with hepatitis B virus, influenza A virus, influenza B virus, herpes simplex virus, human cytomegalovirus, adenovirus, Haemophilus influenzae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Neisseria spp., Staphylococcus aureus, Klebsiella pneumoniae, enterovirus EV71, or the human genome. |
| Compatible Platform | FQD-96A, FQD-96C, FQD-A1600, FQD-A9600 |
| Time | 35 min |
| Recommended Extraction Kit | BSC71 MagaBio plus Virus DNA/RNA Purification Kit II BSC86 MagaBio Plus Virus DNA/RNA Purification Kit III BSC110 MagaBio plus Virus DNA/RNA Purification Kit Ⅵ |
Ordering
| Cat# | Product Name | Packing size |
|---|---|---|
| BSJ90S1 | Dengue Virus/Zika Virus/Chikungunya Virus Nucleic Acid Detection Kit (Fluorescence PCR) | 24T |
| BSJ90M1 | Dengue Virus/Zika Virus/Chikungunya Virus Nucleic Acid Detection Kit (Fluorescence PCR) | 48T |
| BSJ90L1 | Dengue Virus/Zika Virus/Chikungunya Virus Nucleic Acid Detection Kit (Fluorescence PCR) | 96T |