Diarrheagenic Escherichia Coli 5-subtypes Multiplex Detection Kit (Fluorescence PCR)
Details
Product Description
This assay refers to the national standard, selecting different conserved regions of virulence genes for the five Diarrheagenic Escherichia coli, designing specific primers, and synthesizing specific Taqman probes labeled with fluorescent groups. Compared to conventional PCR, this method is characterized by a high level of automation, rapidity, sensitivity, and high specificity. Additionally, a non-competitive exogenous internal standard is introduced during the extraction process to monitor the entire extraction and detection stages, ensuring more accurate and reliable detection results. The aim is to establish a fluorescent quantitative PCR detection method for the rapid detection of the five types of Diarrheagenic Escherichia coli in various foods.
Product Features
- High Accuracy: Effectively detects five virulence genes of Diarrheagenic Escherichia coli, and the test results align with expectations.
- Excellent Specificity: No cross-reactivity observed with other bacteria such as Monophasic Salmonella and Staphylococcus Aureus.
- Real-time Monitoring: Incorporates an exogenous internal control for real-time monitoring throughout the entire extraction and PCR testing process.
- Simple Operation: The entire amplification and testing process is conducted in closed tubes, effectively preventing aerosol contamination.
Application Field
The Diarrheagenic Escherichia coli 5-subtypes Multiplex Detection Kit (Fluorescence PCR) is widely utilized in clinical diagnostics, epidemiological studies, and food safety testing. It enables rapid and accurate detection of five subtypes of diarrheagenic Escherichia coli (DEC), including enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enterohemorrhagic E. coli (EHEC), and enteroaggregative E. coli (EAEC). This kit plays a crucial role in identifying and characterizing DEC infections, tracking outbreaks, and ensuring the safety of food products, thereby contributing to public health and safety.
Storage Conditions
1. The kit need to be transported under freezing conditions.
2. The kit should be stored at -20℃ away from light, and avoid repeated freeze-thaw more than 5 times.
3. The kit can be stored up to 12 months, using the kit in the period of validity.
4. The manufacture and expiration date are shown on the outer packaging.
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