Enterovirus Universal Nucleic Acid Detection Kit (Fluorescent PCR)
Enterovirus belongs to the genus Enterovirus in the family Picornaviridae, consisting of a single-stranded, positive-sense RNA genome approximately 7.2–8.4 kb in length. The genome is divided into three regions, with conserved non-coding regions at both ends, showing significant homology among enteroviruses. The 5' untranslated region is often used as the target gene for universal detection of enterovirus.
Details
Product Description
The Enterovirus Universal Nucleic Acid Detection Kit (Fluorescent PCR) is designed for the qualitative detection of enterovirus nucleic acids in samples. Based on real-time PCR technology, the kit enables sensitive and specific detection of conserved regions shared by enteroviruses. The optimized reaction system provides reliable and reproducible amplification performance. With a simple workflow and convenient operation, the kit offers an efficient solution for enterovirus-related research, epidemiological studies, and public health research.
Product Features
- High Throughput and Broad Range:
This kit supports high-throughput detection, enabling the simultaneous testing of up to 94 samples in a single 96-well experiment.
- Short Detection Time:
Results can be obtained within 1 hour.
- No Risk of Contamination:
The fully closed-tube operation minimizes contamination risk.
- Sample Quality Monitoring:
The non-competitive human β-Globin internal control assesses sample quality and PCR inhibition factors, helping prevent false-negative results.
Storage Conditions
Store at -20 ± 5 ℃, protected from light.
Specification
| Sample Type | Throat Swab |
|---|---|
| Sensitivity | 200 copies/mL |
| Precision | The coefficients of variation for inter-batch, intra-batch, inter-day, and intra-day precision are all less than 5%. |
| Accuracy | Testing with positive reference samples from the company shows a positive agreement rate of 100%, and testing with negative reference samples shows a negative agreement rate of 100%. |
| Specificity | This kit shows no cross-reactivity with norovirus, herpes simplex virus (1/2), enteric adenovirus (40/41), varicella-zoster virus, group A rotavirus, EB virus, rubella virus, measles virus, influenza A virus, parainfluenza virus (1/2/3), influenza B virus, human cytomegalovirus, respiratory syncytial virus (A/B), group B streptococcus, Klebsiella pneumoniae, Escherichia coli, Streptococcus pneumoniae, Staphylococcus aureus, Salmonella, Moraxella catarrhalis, Haemophilus influenzae, Mycoplasma pneumoniae, Bordetella pertussis, adenovirus (3/4/7), or human genomic DNA. |
| Compatible Platform | FQD-96A, FQD-96C, Fully Automatic Nucleic Acid Purification and Real-Time Fluorescence PCR Analysis System (Model: FQD-A1600), ABI7500 |
| Recommended Extraction Kits | BSC71 MagaBio plus Virus DNA/RNA Purification Kit Ⅱ BSC86 MagaBio plus Virus DNA/RNA Purification Kit Ⅲ BSC110 MagaBio plus Virus DNA/RNA Purification Kit Ⅵ |
| Time | 50 min |
| Certificate | RUO |
| Storage Condition | -20 ± 5 ℃ |
Ordering
| Cat# | Product Name | Packing size |
|---|---|---|
| BSJ29M1 | Enterovirus Universal Nucleic Acid Detection Kit (Fluorescent PCR) | 48T |
| BSJ29L1 | Enterovirus Universal Nucleic Acid Detection Kit (Fluorescent PCR) | 96T |