Hop Latent Viroid, Tobacco Mosaic Virus, Cannabis Cryptic Virus Nucleic Acid Detection Kit (Fluorescence PCR）
Hop latent viroid (HpLVd) belongs to the genus Cocadviroid. While it typically causes no obvious symptoms in infected hops, some cultivars may exhibit chlorosis and stunted growth. Infection with this viroid can alter certain secondary metabolites in hops, affecting their quality and reducing yield. Tobacco mosaic virus (TMV), an RNA virus and the causative agent of tobacco mosaic disease, belongs to the genus Tobamovirus and is one of the most widespread diseases affecting tobacco production. Cannabis cryptic virus (CanCV), a double-stranded RNA virus, belongs to the family Partitiviridae.
Details
Product Description
This kit is designed for the qualitative detection of Hop Latent Viroid, Tobacco Mosaic Virus, and Cannabis Cryptic Virus in plant tissues by targeting conserved regions specific to each pathogen for primer and Taqman probe design. A non-competitive internal control is included to monitor sample collection, nucleic acid extraction, and the PCR process, ensuring the reliability of the detection results.
Product Features
Ease of Use
Requires only a single nucleic acid extraction to simultaneously detect three plant viral pathogens.
High Specificity
Demonstrates no cross-reactivity with other pathogens, including Melon Mosaic Virus, Cucumber Mosaic Virus, and Cucumber Green Mottle Mosaic Virus.
Real-Time Monitoring
Incorporates a non-competitive internal control to monitor the entire extraction and detection process.
Contamination Prevention
The kit includes a UDG enzyme-based anti-contamination system to degrade potential contaminant products, preventing false-positive results.
Storage Conditions
-20 ± 5℃
Specification
| Sample Type | Plant tissue |
|---|---|
| Targets | Hop latent viroid (HpLVd), Tobacco Mosaic Virus, Cannabis Cryptic Virus |
| Sensitivity | 500 copies/mL |
| Precision | The coefficients of variation for inter-batch, intra-batch, inter-day, and intra-day precision are all less than 5%. |
| Accuracy | The positive and negative concordance rates for detecting company reference samples are both 100%. |
| Specificity | No cross-reactivity with pathogens such as Melon Mosaic Virus, Cucumber Mosaic Virus, or Cucumber Green Mottle Mosaic Virus. |
| Compatible Platform | LineGene 9600 Plus (FQD-96A) , QuantGene 9600 (FQD-96C), ABI QuantStudio series Real-Time PCR systems. |
| Time | 60 min |
| Recommended Extraction Kits | BSC71 MagaBio plus Virus DNA/RNA Purification Kit II BSC86 MagaBio plus Virus DNA/RNA Purification Kit III BSC77 Biospin Virus DNA/RNA Extraction Kit |
| Storage Condition | -20 ± 5 ℃ |
Ordering
| Cat# | Product Name | Packing size |
|---|---|---|
| BSB99S1 | Hop Latent Viroid, Tobacco Mosaic Virus, Cannabis Cryptic Virus Nucleic Acid Detection Kit (Fluorescence PCR） | 24T |
| BSB99M1 | Hop Latent Viroid, Tobacco Mosaic Virus, Cannabis Cryptic Virus Nucleic Acid Detection Kit (Fluorescence PCR） | 48T |