
QuantFlex ssDNA Quantification Kit
The QuantFlex ssDNA Quantification Kit makes single-stranded DNA quantitation easy and accurate. Depending on sample volume, the assay is accurate for initial sample concentrations from 10 pg/µL to 240 ng/µL. Common contaminants such as salts, solvents, detergents, or protein are well tolerated in the assay. The assay detects oligonucleotides or long ssDNA. Nucleotides and short oligonucleotides of six bases or less do not interfere in the quantitation assay.
Details
Product Description
The QuantFlex ssDNA Quantification Kit provides a rapid, convenient, and highly accurate method for the quantification of single-stranded DNA (ssDNA) or oligonucleotides. Designed for use with the QuantFlex 800 Fluorometer or Qubit Fluorometer, this assay delivers reliable and sensitive detection across a broad initial concentration range of 10 pg/µL to 240 ng/µL, depending on sample input volume.
The assay demonstrates excellent tolerance to common contaminants, including salts, solvents, detergents, and proteins, ensuring accurate results even in complex sample matrices. It is suitable for quantifying both oligonucleotides and long single-stranded DNA. Free nucleotides and short single-stranded DNA fragments (≤6 bases) do not interfere with quantification.
Product Features
Easy to Use: Ready-to-use working solution. Simply mix with sample and incubate. No dye reconstitution required.
High Sensitivity: Excellent linearity from 10 pg/µL to 240 ng/µL with a wide dynamic range.
Inhibitor Tolerant: Resistant to common contaminants such as salts, solvents, detergents, and proteins.
Broad Compatibility: Suitable for ssDNA quantification from various sample sources.
Application Field
The QuantFlex ssDNA Quantification Kit is ideal for quantifying single-stranded DNA (ssDNA), including oligonucleotides and circularized single-stranded DNA (ssDNA) libraries. It is particularly suitable for quantifying circularized ssDNA libraries used in MGI sequencing workflows.
Storage Conditions
1. The kit can be transported at -25℃~8℃.
2. The kit should be stored at 2-8℃ away from light.
3. The kits are stable for 12 month when stored as directed.
4. The production and expiration dates are indicated on the product packaging.
Specification
| Parameters | Description |
|---|---|
| Detection Method | Fluorescence |
| Initial Sample Volume | 1-20 μL |
| Quantitation Range | 10 pg/µL~240 ng/µL |
| Initial Sample Concentration | 0.2-240ng |
| Compatible Platform | QuantFlex 800, Qubit 2, Qubit 3, Qubit 4, and Qubit Flex |
| Compatible Consumables | BSH11S1-L 0.2mL PCR 8-Strip Tubes (With Attached Flat Caps) |
| Storage Condition | Store at 2–8℃, protected from light |
Ordering
| Cat. No. | Product Name | Package |
|---|---|---|
| BSB116S1 | QuantFlex ssDNA Quantification Kit | 250T |
| BSB116M1 | QuantFlex ssDNA Quantification Kit | 1250T |
