
Respiratory Syncytial Virus / Adenovirus / Parainfluenza Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Respiratory Syncytial Virus / Adenovirus / Parainfluenza virus Nucleic Acid Detection Kit (Fluorescence PCR) is used for the qualitative detection of the Respiratory Syncytial Virus (RSV), Adenovirus (ADV) and Parainfluenza virus (PIV) nucleic acid in specimens of oropharyngeal swabs from suspected cases.
Details
Product Description
This product selects highly conserved regions of Respiratory Syncytial Virus, Adenovirus and Parainfluenza virus to designs specific primers and fluorescent probes. The 5' end of the probe is labeled with the fluorescence reporter and the 3' end is labeled with the fluorescence quencher. During PCR amplification, when the probe maintains the complete sequence, the fluorescence of the fluorescence reporter is absorbed by the fluorescence quencher without producing fluorescence.
In order to control the whole process of sampling, extraction and PCR amplification, human gene was act as a non-competitive internal control during the detection process.
Product Features
Multiplex Detection:
3 in 1 test for Respiratory Syncytial Virus (RSV), Adenovirus (ADV), Parainfluenza Virus (PIV).
Short Turnaround Time:
Results can be obtained in as little as 35 minutes.
Internal Control Included:
Incorporates an internal control to monitor the entire extraction and detection process, ensuring reliability and quality of the results.
Easy to Use:
qPCR is completed in a single step with fully enclosed amplification and detection, preventing aerosol contamination.
Application Field
The Kit (Fluorescence PCR) is used for the qualitative detection of the Respiratory Syncytial Virus (RSV), Adenovirus (ADV) and Parainfluenza virus (PIV) nucleic acid in specimens of oropharyngeal swabs from suspected cases.
Storage Conditions
1. The unopened kit should be stored at -20℃±5℃ away from light.
2. The kit can be stored for up to 12 months if all components are kept in the manner above. Do not use after the stated expiry date.
3. The kit can be transported for 7 days at temperatures not exceeding 8°C without affecting its validity period.
4. Don’t open unless you are ready to use the kit. The kit can be stored for 7 days at 2~8℃ after opening.
5. Avoid repeated freeze-thaw (no more than 5 times).
Specification
| Sample type | oropharyngeal swab |
|---|---|
| Target Pathogens | Adenovirus (FAM)Respiratory Syncytial Virus (HEX)Parainfluenza Virus (ROX) |
| Precision | The variation coefficient (CV) of within-day, between-day, within-batch and between-batch were less than 5% |
| Detection Time | 35 min |
| Sensitivity | 500 copies/mL |
| Accuracy | It can simultaneously detect RSV, ADV, PIV |
| Compatible Instruments | Bioer’s Fluorescence Quantitative Detection System, LineGene 9600 Plus (FQD-96A) and QuantGene 9600(FQD-96C), Applied Biosystems 7500 Real-Time PCR Systems. |
| Recommended Extraction Kit | BSC86 MagaBio Plus Virus DNA/RNA Purification Kit III BSC71 MagaBio Plus Virus DNA/RNA Purification Kit II |
| Certificate | CE-IVD |
Ordering
| Cat# | Product Name | Packing Size |
|---|---|---|
| BSJ45M1 | Respiratory Syncytial Virus / Adenovirus / Parainfluenza Virus Nucleic Acid Detection Kit (Fluorescence PCR) | 48T |
| BSJ45L1 | Respiratory Syncytial Virus / Adenovirus / Parainfluenza Virus Nucleic Acid Detection Kit (Fluorescence PCR) | 96T |
