STI-7 Nucleic Acid Detection Kit (Fluorescence PCR)
Details
Product Description
This assay targets conserved genomic regions of seven STI pathogens, utilizing specifically designed primers and fluorescently labeled TaqMan probes for each. Compared to conventional PCR, this method offers higher automation, speed, sensitivity, and specificity. The assay employs an optimized reaction buffer system and hot-start DNA polymerase to produce a ready-to-use master mix, minimizing manual preparation steps and enhancing operational efficiency. In addition, the human RNase P gene is introduced as a non-competitive internal control to monitor both nucleic acid extraction and amplification processes, effectively preventing false-negative results.
Product Features
- All-in-One Premix
Ready-to-use master mix requires only the addition of sample, significantly reducing hands-on time and minimizing manual errors.
- Contamination Control
Incorporates a dUTP/UDG system to effectively prevent carryover contamination between reactions.
- High Specificity
No cross-reactivity was observed with a range of non-target pathogens, including Group B Streptococcus, Human Papillomavirus (HPV), Salmonella spp., Pseudomonas aeruginosa, Escherichia coli, Treponema pallidum, Human Cytomegalovirus (CMV), Epstein-Barr Virus (EBV), Human Herpesvirus 6 (HHV-6), Candida albicans, Mycoplasma pneumoniae, Bordetella pertussis, Staphylococcus aureus, Candida glabrata, and Adenovirus.
- Real-time Monitoring
The human RNase P gene is incorporated as a non-competitive internal control to monitor the entire nucleic acid extraction and amplification process, ensuring reliability and minimizing the risk of false-negative results.
Storage Conditions
-20 ± 5℃, protected from light
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