Streptococcus Pneumoniae and Haemophilus Influenzae Nucleic Acid Detection Kit (Fluorescence PCR)

Product Description

This kit selects highly conserved regions of Streptococcus pneumoniae and Haemophilus influenzae, and designs specific primer probes. The primers and probes can specifically bind to the target sequence, and under the action of Taq enzyme, the fragment can be specifically amplified, and the fluorescent Probes are hydrolyzed during amplification, resulting in fluorescence. The detection system contains dUTP-UDG enzyme anti-pollution measures to fully degrade possible product contamination and avoid false positive results; in addition, an internal standard is set, which is a highly conserved region on the human genome, and the internal standard monitors sample collection, transportation, nucleic acid extraction and PCR amplification. Increase the whole process, avoid false negative results, and ensure the effectiveness of the whole process.

Product Features

- Dual-Target Detection: Simultaneous detection of Streptococcus pneumoniae and Haemophilus influenzae.

- Rapid Detection: Results available in only 32 minutes.

- Contamination Control: dUTP-UDG system minimizes carryover contamination and false-positive results.

- Full-Process Monitoring: Human internal control monitors the entire workflow to reduce false-negative results.

Application Field

Streptococcus Pneumoniae and Haemophilus Influenzae Nucleic Acid DetectionKit (Fluorescence PCR) is used for the qualitative detection of the Streptococcus Pneumoniae (SP) and Haemophilus Influenzae (Hl) nucleic acid in specimens ofsputum from suspected cases. The kit is used for the auxiliary diagnosis and epidemiological surveillance of SP and HI infection, cannot be used as the basis for the diagnosis or exclusion of cases alone.

For professional use only.

For in vitro diagnostic use only.

Application Cases

1. Accuracy: after the positive reference products P1-P10 and negative reference products N1-N10 of Hangzhou Bioer Technolo gy Co., Ltd. are reconstituted as required, they are extracted with Bioer extraction kit BSC71S1E and tested.

Result: The results show that the nucleic acid of Streptococcus pneumoniae and Haemophilus influenzae can be accurately detected. The test results of enterprise reference products: the positive coincidence rate is 100%, and the negative coincidence rate is 100%.

2. Precision: after the precision reference products J1-J2 of Streptococcus pneumoniae and Haemophilus influenzae Nucleic Acid Detection Kit (Fluorescence PCR) are reconstituted according to the requirements for use and extracted by Bioer extraction kit BSC71S1E, using three batches of the detection kit to do the detection, and repeated for 10 times.

Result: The results show that the inter-assay and intra-assay precision variation coefficients of the three batches of detection kit were all less than 5%, and the reagent precision is great.

3. Clinical specimen experiment: Bioer extraction kit BSC71S1E is used to extract and detect clinical specimens of Streptococcus pneumoniae and Haemophilus influenzae. At the same time, the detection kit of the same kind that have obtained NMPA certificate are used as a comparison to verify the compliance rate.

Result: The results show that: compared with the competing product of Streptococcus pneumoniae and Haemophilus influenzae detection kits, the clinical samples verified by Bioer Streptococcus pneumoniae and Haemophilus influenzae detection kit has a higher detection coincidence rate and higher amplification efficiency.

Storage Conditions

1. The kit should be stored at -20C°C away from light.

2. The kit can be stored for up to 12 months if all components are kept in the manner above. Do not use after the stated expiry date.

3. The kit can be transported in foam box sealed with ice bags or dry ice under -8°C for 7 days.

4. After opening, the kit can be stored at 2°C-8°C for 7 days.

5. Avoid repeated freeze-thawing, and the number of freeze-thawing should not exceed 5 times.