Multiplex qPCR Probe Master Mix I (UDG plus) (2×) is designed as an ready-to-use solution for detection and quantitation of target DNA sequences using hydrolysis probes. The combination of optimized buffer, hot-start Taq DNA polymerase and stabilizers results in high amplification efficiency, high detection sensitivity and less non-specific amplification in real-time PCR analyses. This pre-mixed formulation allows us just adding primers, probes and templates, which reduce number of steps and save time. The hot-start Taq DNA Polymerase is a mixture of Taq DNA polymerase and Taq antibody. The antibody binds to the enzyme and inhibits its activity at temperatures up to 50°C, while the enzyme activity is released during initial denaturation allowing reactions to be set up at room temperature. This master mix retains Uracil-DNA Glycosylase (UDG) catalyses the release of free uracil from uracil-containing PCR products from previous amplifications or non-specific products.
- Polymerase Chain Reaction (PCR)
- Site-Directed Mutagenesis
- Cloning
- Sequencing
- Genotyping
- Gene Expression Analysis
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